货号价格

JAG-HM101-100μg / 询价

JAG-HM101-500μg / 询价

JAG-HM101-500μgx2 / 询价

Human Jagged 1/JAG1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Jagged 1/JAG1 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln34-Ser1046 [Accession | P78504-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 111.56 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Jagged 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

背景(Background)

JAGGED1 (JAG1) is composed of a relatively small intracellular domain, a transmembrane domain and a larger extracellular component. JAG1 is widely expressed throughout mammalian development, across many tissues and developmental stages. The Notch signaling pathway and JAG1 in particular have been implicated in multiple forms of cancer and multiple aspects of cancer biology including tumor angiogenesis, neoplastic cell growth, cancer stem cell maintenance and the metastatic process.

分子别名(Synonyms)

Jagged1; hJ1; CD339; JAGL1; AGS; AHD; AWS

文献(References)

Grochowski CM, Loomes KM, Spinner NB. Jagged1 (JAG1): Structure, expression, and disease associations. Gene. 2016 Jan 15;576(1 Pt 3):381-4. doi: 10.1016/j.gene.2015.10.065. Epub 2015 Nov 6. PMID: 26548814; PMCID: PMC4673022.