货号价格

HGF-HM101-100μg / 询价

HGF-HM101-500μg / 询价

HGF-HM101-500μgx2 / 询价

Human HGF Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln32-Ser728 [Accession | P14210-1].

分子量大小(Molecular Weight)

The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human HGF, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

背景(Background)

Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.

分子别名(Synonyms)

Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

文献(References)

(1) Oliveira AG, Araújo TG, Carvalho BM, Rocha GZ, Santos A, Saad MJA. The Role of Hepatocyte Growth Factor (HGF) in Insulin Resistance and Diabetes. Front Endocrinol (Lausanne). 2018 Aug 30;9:503. doi: 10.3389/fendo.2018.00503. PMID: 30214428; PMCID: PMC6125308.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.