货号价格

GDF-HE001-100μg / 询价

GDF-HE001-500μg / 询价

GDF-HE001-500μgx2 / 询价

Human GDNF Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GDNF Protein is expressed from E.coli without tag
It contains Ser78-Ile211 [Accession | P39905-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GDNF, No Tag at 1 μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA.

背景(Background)

Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

分子别名(Synonyms)

Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)

文献(References)

Cintrón-Colón AF, Almeida-Alves G, Boynton AM, Spitsbergen JM. GDNF synthesis, signaling, and retrograde transport in motor neurons. Cell Tissue Res. 2020 Oct;382(1):47-56. doi: 10.1007/s00441-020-03287-6. Epub 2020 Sep 8. PMID: 32897420; PMCID: PMC7529617.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.