EGF-MM202-100μg / 询价
EGF-MM202-500μg / 询价
EGF-MM202-500μgx2 / 询价
Mouse EGF Protein
Recombinant Mouse EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Asn977-Arg1029.[Accession | P01132]
The protein has a predicted MW of 32.35 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse EGF is greater than 95% as determined by SEC-HPLC.
Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 9.96 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.4 - 3 ng/mL.
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
HOMG4; EGF; URG
(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.
