EGF-HM202-100μg / 询价
EGF-HM202-500μg / 询价
EGF-HM202-500μgx2 / 询价
Human EGF Protein
Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Asn971-Arg1023.[Accession | P01133-1]
The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL (QC Test).
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
HOMG4; EGF; URG
(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.
