Human M-CSF/CSF-1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag.
It contains Glu33-Asn190 [Accession | P09603-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.40 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.05EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8ng/ml determined by ELISA (QC Test).

SPR Data

Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL (QC Test).

背景(Background)

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

分子别名(Synonyms)

CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim

文献(References)

(1)Cannarile M A , Weisser M , Jacob W , et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.