COV-VM1SD-100μg / 询价
COV-VM1SD-500μg / 询价
COV-VM1SD-500μgx2 / 询价
SARS-COV-2 Spike S1 (Deltacron) Protein
Recombinant SARS-COV-2 Spike S1(Deltacron) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Alanine substitutions (R683A) are introduced to stabilize the trimeric prefusion state of SARS-COV-2 Spike S1(Deltacron) Protein and abolish the furin cleavage site, respectively.[Accession | QHD43416.1]
The protein has a predicted MW of 73.94 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike S1 (Deltacron) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
