Human ANGPTL4/Angiopoietin-like 4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Pro166-Ser406.[Accession | Q9BY76-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Angiopoietin-like 4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Angiopoietin-like 4 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human ANGPTL4, His Tag with an affinity constant of 24.50 nM as determined in SPR assay (Biacore T200).

背景(Background)

Candidates for this common regulatory system include signals mediated by peroxisome proliferator-activated regulator and its response factor, angiopoietin-like 4. The expression and bioactivity of angiopoietin-like 4, an adipocytokine that was originally reported to have an angiogenic function, have been detected not only in the vascular system and adipose tissue but also in rheumatoid joints.

分子别名(Synonyms)

ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4;

文献(References)

(1) Masuko K. Angiopoietin-like 4: A molecular link between insulin resistance and rheumatoid arthritis. J Orthop Res. 2017 May;35(5):939-943. doi: 10.1002/jor.23507. Epub 2017 Mar 8. PMID: 28004425.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.