货号价格

NKC-HM495B-100μg / 询价

NKC-HM495B-500μg / 询价

NKC-HM495B-500μgx2 / 询价

Biotinylated Human NKG2A&CD94 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)]

分子量大小(Molecular Weight)

The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human NKG2A&CD94, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.

背景(Background)

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

分子别名(Synonyms)

CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

文献(References)

(1) Hò GT, Celik AA, Huyton T, Hiemisch W, Blasczyk R, Simper GS, Bade-Doeding C. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain. Int J Mol Sci. 2020 Jun 19;21(12):4362. doi: 10.3390/ijms21124362. PMID: 32575403; PMCID: PMC7352787.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.