NEC-HM401-100μg / 询价
NEC-HM401-500μg / 询价
NEC-HM401-500μgx2 / 询价
Human Nectin-1/PVRL1/CD111 Protein
Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln31-Thr334.[Accession | Q15223-1]
The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Nectin-3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24μg/ml determined by ELISA (QC Test).
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).
Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200).
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR
(1)Yu Z , Adusumilli P S , Eisenberg D P , et al. Nectin-1 Expression by Squamous Cell Carcinoma is a Predictor of Herpes Oncolytic Sensitivity[J]. Molecular Therapy, 2007, 15(1):103-113.
